Fetal Bovine Serum(FBS) IgG Depleted 

Catalog Number S 001-09 

Storage Temperature -20°C

제품설명

일반 FBS에는 IgG가 약 70–300 µg/mL 수준으로 존재하지만, 웰진은 고품질 FBS를 원료로 제작된 IgG 제거 FBS를 공급합니다. 

이 제품은 크로마토그래피 공정을 통해 IgG를 효과적으로 제거하여 최종 IgG 농도를 5 µg/mL 미만으로 낮춘 제품입니다. 

IgG 제거 후에도 세포 성장 성능은 일반 FBS와 동등하게 유지됩니다.

· 총 IgG 함량 5 µg/mL 이하

· 단클론 항체 생산에 우수한 성능

· 0.1 µm 3회 필터링

· 1000L 이상 대용량 롯트 공급 가능

· 모든 FBS는 완전 추적 가능하며, 검증된 공급업체로부터 공급

IgG 제거 FBS를 사용하는 이유는?

IgG가 제거된 혈청은 다양한 연구 및 임상 응용에서 여러 장점을 제공합니다.
면역측정법(예: ELISA)에서 높은 수준의 IgG는 비특이적 결합 및 위양성 반응을 유발할 수 있는데, IgG 제거 혈청은 이러한 배경 간섭을 줄여줍니다.

이 혈청은 세포배양 및 항체 생산에도 유용하여 교차 반응 위험을 최소화하고 항원-항체 상호작용의 특이성을 향상합니다. 또한 내인성 IgG의 영향을 제거함으로써 면역학 연구에서 실험 결과의 정확성과 신뢰도를 향상시킵니다.

더 나아가, IgG 제거 혈청은 특정 백신 및 치료 단백질 생산에 필수적이며 최종 제품의 순도와 효능을 보장합니다.

Proper storage and thawing method of FBS

1. After FBS thawing, why does clots of fleece shape appear? And how affect it to cell culture?

During FBS thawing, the most important thing is to shake slowly every 5~10 minutes until thawed all, minimizing bubbles.

If the process does not work correctly, concentration gradient with insoluble crystals of fleece shape(cryoprecipitates) can be generated result from imbalance of salt and protein.

The cryoprecipitates is little effect on cell culture. In particular, thawed FBS should not be refrigerated and if you don’t follow the precautions, the clots can be caused a lot of further.

Because of this, aliquots of thawing must be frozen keep. The cryoprecipitates is not a defect or fault of product.

2. What is proper storage and use method of FBS?

1) Proper FBS thawing process(for minimizing deposits)

While FBS thawing, shake it slowly every 5 ~ 10  minutes minimizing bubbles for breaking concentration gradient.

· Fill water in water bath almost to top of FBS(except cap) and raise temperature of water both by 37℃..

· Put frozen FBS in water bath and thaw it by shaking slowly ever 5~10 minutes until breaking concentration gradient.  

· After thawing all, take out and spray 70% alcohol on the surface of bottle.

· Divide FBS into 20~100ml in clean bench.

· Freeze divided FBS as soon as possible. And keep FBS in refrigerator if it is used in a day or two. But keep in mind FBS kept in refrigerator can generate more clots than one in freezer.

· If Heat-Inactivation process is needed, proceed as follows (in case of buying products that Heat-Inactivation completed, this process is unnecessary)

   - Put thawed FBS in 56℃ water bath and proceed Heat-Inactivation for 30 minutes.(Optional; heat-inactivation is unnecessary for most cell culture applications.)

  - Shake FBS slowly every 5~10 minutes minimizing bubbles and not making concentration gradient like thawing process. If this Heat-inactivation proceeded irregularly, lots of clots can be generated than thawing process.

2) Must storage FBS in a freezer without automatic frost-free function. (Avoid storage in a home refrigerator/freezer, which has frost-free function.)

Must Storage in a freezer without automatic frost-free function for preventing deterioration of FBS from temperature change.  A freezer with automatic frost-free function change the temperature automatically for preventing frost, so it can cause reduce the quality of FBS by freezing and thawing the surface of product repeatedly. And give attention that product bottle can be broken in temperature. 

3) Minimize freezing-thawing process

Temperature change from freezing-thawing process destroys protein, growth factor, hormone and other nutrient. Thus, it is necessary to minimize freezing-thawing process. After first thawing, divide FBS into amount used to once or twice(20~100ml) and frozen. This can reduce iterative freezing-thawing process and thawing hours. At this time attention not to exceed maximum volume 40ml in 50ml conical tube and 90ml in 100ml glass bottle considering of FBS expands as it freezes.