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10 mg/ml Ethidium Bromide (EtBr) Solution

  10 mg/ml Ethidium Bromide (EtBr) Solution
1. 0.1 g의 EtBr을 10 ml의 물에 녹인다.
2. 수 시간동안 교반하여 완전히 녹인다.
3. 갈색 병 또는 aluminum foil을 사용하여 빛을 차단한 채로 상온에서 보관한다.
Notes : EtBr은 매우 강력한 돌연변이 유도물질(mutagen)이므로 반드시 비닐장갑과 마스크를 착용하고 chemical hood에서
작업한다.


Technical Tables for Molecular Biology
Acrylamide Gel Electrophoresis of Xylene Cyanole FF and Bromophen..
Acrylamide Gel Electrophoresis of Proteins
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Amino Acids
Amino Acids
Antibiotic Solutions
Genetic Markers
Genotypes
Nucleic Acid Data
pH of 50 mM Tris-HCl Buffer Solutions
Physical Constants of the Nucleoside Triphosphates
Recommended Gel Percentages for Separation of Linear DNA
Specifications of Common Vectors


Enzymes for DNA and RNA Modification
DNA Polymerases
Ligases
Nucleases
Phosphatases
Reverse Transcriptases
RNA Polymerases
Others


Protocols
DNA Mini-Agarose Gel Electrophoresis
Transformation of E. coli (CaCl2 and heat-shock method)
Mini-Prep of Plasmid DNA (Alkaline Lysis Method)


Preparation of Liquid solutions and Buffers
45% Acrylamide Solution
Actinomycin D Solution
0.1 M Adenosine 5'-Triphosphate (ATP) Solution
10 M Ammonium Acetate Solution
10% Ammonium Persulfate (APS) Solution
20 mg/ml 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside (X-Gal..
50 mg/ml 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) Solution
1 M CaCl2 Solution
50 mM Deoxyribonucleoside Triphosphate (dNTP) Solutions (Each)
1 M Dithiothreitol (DTT) Solution
10 mg/ml Ethidium Bromide (EtBr) Solution
0.5 M Ethylenediaminetetraacetic Acid (EDTA) Solution, pH 8.0
200 mg/ml Isopropylthio-β-D-Galactoside (IPTG) Solution
50 mg/ml Nitro Blue Tetrazolium (NBT) Solution
Phenol Equilibration
Phosphate-Buffered Saline (PBS) Solution
Potassium Acetate Solution(For Alkaline Lysis Method of Plasmid P..
20X Saline-Sodium Citrate (SSC) Buffer
20X Saline-Sodium Phosphate-EDTA (SSPE) Buffer
3 M Sodium Acetate (pH 5.2 and pH 7.0) Solution
20% Sodium Dodecyl Sulfate (SDS, Lauryl Sulfate Sodium Salt) Solu..
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