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Specifications of Common Vectors

Name Marker* Promoters Size(kb) GeneBank Classification
 pUC19  Ac,BW    2.7  L09137  High Copy
 pBluescript II KS+/- (SK+/-)  Ap, BW  T3, T7  3  X52327  High Copy
 pGEM 3Zf(+/-)  Ap, BW  SP6, T7  3.2  X65306/7  High Copy
 pT7Blue  Ap, BW  T7  2.9    High Copy
 pBR322  Ap, Tc    4.4  L08654  Low Copy
 pACYC184  Cm, Tc    4.2    Low Copy
 M13mp18 (8, 9, 19, BM20/21)  BW    7,2  UX02513  Low Copy
 pWE15  Ap, neo  T3, T7  8.2    Low Copy
 SuperCos  Ap, neo  T3, T7  7.6    Low Copy
*Ap - Ampicillin Resistance, BW - Blue/White Selection, Cm - Chloramphenicol Resistance, neo - Aminoglydoside
  3'-phosphotransferase II gene (encodes resistance to neomycin), Tc - Tetracycline Resistance.

Technical Tables for Molecular Biology
Acrylamide Gel Electrophoresis of Xylene Cyanole FF and Bromophen..
Acrylamide Gel Electrophoresis of Proteins
Agarose Gel Electrophoresis of Xylene Cyanole FF and Bromophenol ..
Amino Acids
Amino Acids
Antibiotic Solutions
Genetic Markers
Nucleic Acid Data
pH of 50 mM Tris-HCl Buffer Solutions
Physical Constants of the Nucleoside Triphosphates
Recommended Gel Percentages for Separation of Linear DNA
Specifications of Common Vectors

Enzymes for DNA and RNA Modification
DNA Polymerases
Reverse Transcriptases
RNA Polymerases

DNA Mini-Agarose Gel Electrophoresis
Transformation of E. coli (CaCl2 and heat-shock method)
Mini-Prep of Plasmid DNA (Alkaline Lysis Method)

Preparation of Liquid solutions and Buffers
45% Acrylamide Solution
Actinomycin D Solution
0.1 M Adenosine 5'-Triphosphate (ATP) Solution
10 M Ammonium Acetate Solution
10% Ammonium Persulfate (APS) Solution
20 mg/ml 5-Bromo-4-Chloro-3-Indolyl-β-D-Galactopyranoside (X-Gal..
50 mg/ml 5-Bromo-4-Chloro-3-Indolyl Phosphate (BCIP) Solution
1 M CaCl2 Solution
50 mM Deoxyribonucleoside Triphosphate (dNTP) Solutions (Each)
1 M Dithiothreitol (DTT) Solution
10 mg/ml Ethidium Bromide (EtBr) Solution
0.5 M Ethylenediaminetetraacetic Acid (EDTA) Solution, pH 8.0
200 mg/ml Isopropylthio-β-D-Galactoside (IPTG) Solution
50 mg/ml Nitro Blue Tetrazolium (NBT) Solution
Phenol Equilibration
Phosphate-Buffered Saline (PBS) Solution
Potassium Acetate Solution(For Alkaline Lysis Method of Plasmid P..
20X Saline-Sodium Citrate (SSC) Buffer
20X Saline-Sodium Phosphate-EDTA (SSPE) Buffer
3 M Sodium Acetate (pH 5.2 and pH 7.0) Solution
20% Sodium Dodecyl Sulfate (SDS, Lauryl Sulfate Sodium Salt) Solu..
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